Catalase Enzyme Lab Report Guy Guarino Abstract Enzymes are protein catalyst that speed up the rate of a chemical reaction. Powered by Create your own unique website with customizable. The test tube with the sand as it isn’t living and does not include an enzyme within it. Potato Catalase Lab November 16, 2017 David Swart Leave a comment For the lab today, students were instructed to record the steps of the procedure in their lab notebooks. Pour 6 cm of water into beaker. What do you classify a species that is likely to become endangered if the factors that make it vulnerable are not reversed?. The enzyme catalase speeds up the breakdown of hydrogen peroxide (H202) into water (H2O) and oxygen gas (02). Put soaked potato pieces into test tubes, one for ammonia, one for vinegar, and one for water for 90 seconds. Experiment Result: Work of Catalase Enzyme - Free download as Powerpoint Presentation (. Bar Graph: Potato Catalase Lab. , and Jensen, P. Hydrogen peroxide is produced naturally in organisms, but is very harmful. The sweet potato is naturally more sweet, and this caused the results of the reaction to be less noticeable. Explain the chemical reaction from question 2 in words. The interaction of the bubbles with the soap solution produces a good amount of foam. Both pepperoni pizza and donuts contained all the food compounds except Vitamin C. Procedure: Procedure for room temperature potato and frozen potato: Mash a potato piece and add to a test tube. What is Catalase ? Catalase is an enzyme produced by microorganisms that live in oxygen-filled environments to protect themselves from the oxidative damage of hydrogen peroxide (H2O2). Surprisingly, based on our results, the catalase breakdown in a potato works the most quickly in an acidic pH level. Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals). The ground-up potato is filtered through cheesecloth to separate potato skin and cell debris from the liquid which contains most of the cell's enzymes, including catalase. Academic year. Evaluation & Improvements: 1. In this lab, you will study catalase, an enzyme that is found in the cells of many living tissues. The other test tube will contain the enzyme in a masked raw potato. fruit and Jello an hour before the lab begins. Catalase and other enzymes help break hydrogen peroxide into water and oxygen gas. In this lab activity, you will be using yeast catalase, but you could also use catalase from potatoes, carrots, plant leaves, chicken liver, or steak…. The pages at the back of the lab manual give you general instructions for preparing your report. AP Biology Lab 2: Enzyme Catalysis. Conclusion: All my hypotheses were supported with the data I collected in this lab :)! This experiment depicts the importance of enzymes in the human body. Enzyme Catalysis Lab Review Worksheet - Winnie Litten. Use forceps to dip disc into catalase for 5 seconds. We will also keep consistent the type of potato, so that we are observing pH's effect on catalase activity rather than the different types of potatoes' effect on catalase activity. Sometimes, catalase also uses hydrogen peroxide to oxidise contaminants including Phenols, Formic Acidity, Formaldehyde and Alcohols. Some errors in my lab might have been because we didn't put the potato cores in the solutions all at the same time so it might have messed up my data a little bit. Hydrogen peroxide has been known to bleach hair, and low catalase levels have been linked to the onset of graying. Expect two potatoes in series to be able to light an LED; however, you might need three. There are independent variables, dependent variables, contants and controls used in this lab. Microsoft Word - 1-6 Virtual Enzyme Lab. 6 Root pressure Glass tubes are fitted to the cut branches of a potted plant. The New Fuss About Potato Enzyme Lab Report. If we use potato or other tissue containing this enzyme, we can use this to measure the relative influence of varying different factors on the activity of enzymes in living tissue, the factor I will be investigating in my coursework is the activity of pH. Our hypothesis was that hydrogen peroxide would react with the catalase in the potato for the longest duration of time, following after would be ammonia then vinegar. com does not make any guarantee or representation regarding the Science Fair Project Ideas and is not responsible or liable for any loss or damage, directly or indirectly, caused by your use of such information. Obtain 75 ml of 100% potato extract (catalase source) from your teacher. Insert one nail in each potato. Test the boiled (100 oC) catalase. Enzyme Catalysis Lab Review Worksheet - Winnie Litten. We will explore and observe the concept of osmosis, which is when water moves from an area of high water concentration to an area of low water concentration, through a membrane. Catalase activity is a function of aerobic process. Academic year. Potato Enzyme Lab. coli and Streptococcus durans. Luckily, it is also found in potatoes. The enzyme employed within this lab is catalase. Background Information: An enzyme is catalytic protein. The catalase sample for today’s lab will be from a potato. My hypothesis was supported because the "normal environment" which is basically room temperature. Come browse our large digital warehouse of free sample essays. They are long chain amino acids bound together by peptide bonds. As you hold the potato, keep your fingers on the front and your thumb on the back and not on the top and bottom. The purpose of this lab is to observe normal catalase reaction, effect of temperature on catalase, effect of pH on catalase, and identify presence of catalase in tissues other than liver. Annie Chiang. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H 2O 2 hydrogen peroxide Catalyst = peroxide 3. Without catalase, the cells would be damaged by the hydrogen peroxide they produce. Plants and some bacteria also produce amylase. Lab report hypothesis. is a substance that increases the rate of a chemical reaction without being consumed or permanently changed in the reaction. Effect of Environmental Variables on Enzyme Reaction Rate in Living Cells. So we can measure the amount of bubbles to see how well the enzyme catalase worked. The flow of electrons results in the generation of an electric charge. Liver produces more bubbles and foam than the potato. The reaction that took place in the basic ammonia however, had bubbles reaching an average height of 4. In trying to find a cell’s water potential in different molar concentrations and how the concentrations affect a real cell system, we conducted two labs. Scientists know that lab reports are a very important part of every experiment. Investigating macromolecules. OBJECTIVES: 1. During this initial lab catalase will be isolated from potatoes. In this lab activity, you will study the enzyme catalase. Potato catalase work best at a higher pH. Potatoes, particularly, contain high amounts of catalase, which is mysterious because plants do not filter toxins from food. Potato chips were the only food tested that contained all six compounds. The Lab is now accepting proposals for the 2020 cycle. The enzyme employed within this lab is catalase. If I need fake Internet connectivity I set my lab up like this (this is the lab we used for a Direct Access POC today): All of the magic mentioned at the top of this blog happens on the Windows 2008 R2 VM with two network interfaces. 2M solution of sucrose because there was only a 4. Label one clean slide for each organism to be assayed. 1) You will construct a testable hypothesis about the effect of a factor (temperature, pH or substrate. University of South Carolina. The substrate of the enzyme will be 3% hydrogen peroxide (H 2 O 2). If-then statements are used to explore cause and effect. Google Doc Enzyme Lab. Hence the main objective of the project was to undertake a systematic study of the purification and properties of catalase from plant sources such as Solanum tuberosum (Potato plant) and Agaricus. Concentration of H2O2 in Catalase activity? what is the concentration of H2O2 can be use in bacterial catalase enzyme study ? As per of observation few articles mention the direct use if H2O2 from. Amelia Houlihan - Catalase Lab Report. The purpose of this lab was to study what would happen to us if catalase didn't work as well as it does, if we had more acid in our system. Having three different substances allowed us to determine whether each contained catalase. , a potato) is heated, there is less and less active catalase. Both plants and animals have peroxisomes with catalase. is a substance that increases the rate of a chemical reaction without being consumed or permanently changed in the reaction. About This Assay Cayman’s Catalase Assay Kit utilizes the peroxidatic function of CAT for determination of enzyme activity. Make sure you slice the potato down the middle, lengthwise. Experiment Result: Work of Catalase Enzyme - Free download as Powerpoint Presentation (. This is to be done the previous day in class or for a homework assignment the night before. No, during Part A we took used liver and put it in more peroxide. Determine if temperature and pH affect enzyme effectiveness. At equilibrium, the following condition exists: Pure Water: Ψ = Ψ P + Ψ. Enzymes speed up or slow down chemical reactions of the cells. The catalase appears to be affected by fluctuating pH values, and operates most ideally at pH 9. Transcript of Potato and Hydrogen Peroxide Enzyme Lab. 5 Turgor in potato tissue Cylinders of potato tissue are immersed in solutions of differing strength and changes in length are measured. 0144, and K m value of 0. Enzymes are proteins that act as biological catalysts. Catalase neutralizes hydrogen peroxide and turns it into water and oxygen*. Proton Transfer Drives Protein Radical Formation in Helicobacter pylori Catalase but Not in Penicillium vitale Catalase. During this lab activity we will explore the properties of catalase, as well as measure the relative influence of varying several different factors on the. Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the Classic edition of The Biology Place to all educators and their students. This is possible because when a cell dies, the enzymes remain intact and active for several weeks, as long as the tissue is kept refrigerated. The hypothesis that at temperatures above 40°C, the enzyme catalase will become less effective, is proven to be true. The conclusion should address the purpose of the lab and your hypothesis (i. Liver Enzyme Lab Purpose : The purpose of this experiment was to determine the effect of the enzyme catalase found in liver on the decomposition of 6% hydrogen peroxide into water and oxygen gas. ;Assignment Question 4: First, come up with a good hypothesis about how any two of these 10 characteristics might be related to one another. It quickens the process of harmful hydrogen peroxide breaking into harmless components of water and oxygen. Then, we’ll enjoy some examples of hypothesis testing. Procedure: Procedure for room temperature potato and frozen potato: Mash a potato piece and add to a test tube. The enzyme catalase is a heme containing protein, carrying one heme group at each active site. To determine the effects of pH on catalase enzyme activity. 2 Potato cubes Razor blade Procedure 2: Effect of enzyme concentration For this experiment you will use potato as a source of catalase. Get 100% original potato-osmosis lab reports. 4, then the reaction will occur at a slower rate than when catalase is added to hydrogen peroxide with a pH of 4. Understanding Enzymes In the lab "Understanding Enzymes" , we tested the reaction that Enzyme Catalase would have in beef liver, apple, potato and chicken with Hydrogen Peroxide and even water. Instead, it is the presence of a substance called catalase. In this experiment, catalase will be extracted from fresh potato tubers. Hui Chen, Wenzhen Lai, and Sason Shaik. The breakdown of hydrogen peroxide into water and oxygen is catalysed by catalase enzymes in plants and animals. the work of catalase enzyme based on the REAL experiment. What are the products in the chemical reaction when catalase breaks down hydrogen peroxide? 6. BACKGROUND: Catalase (the enzyme) is found in the cells of the liver where it breaks down hydrogen peroxide (the substrate) into water and oxygen according to this equation. An experiment is a test that supports or disproves a hypothesis. Include this information in the introduction portion of your lab report. In this experiment, you well use a potato as a source of catalase. Our goal is to understand and treat human diseases of the blood system and to develop new approaches to treat cancer. Understanding Enzymes In the lab "Understanding Enzymes" , we tested the reaction that Enzyme Catalase would have in beef liver, apple, potato and chicken with Hydrogen Peroxide and even water. The Catalase Question – Experimental Design FORMING A QUESTION AND A HYPOTHESIS Question: Hypothesis: Enzyme Simulation Lab. Add 20 ml of 30% H202 into a beaker. Safety note: oxygen is a highly reactive gas, adult supervision recommended for this project. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below. University of South Carolina. • Pour the 100 oC catalase into a paper cup. Experiment Result: Work of Catalase Enzyme - Free download as Powerpoint Presentation (. But that data is not very useful unless it is organized. It might seem strange to use dead cells to study the function of enzymes. Liver produces more bubbles and foam than the potato. They are responsible for the synthesis and degradation of lipids, amino acids, carbohydrates,. ) One unit of pure catalase decomposes 1 ɥmole H2O2 per minute at 25°C at pH7. Describe what happened biochemically to catalase and hydrogen peroxide during this experiment. The purpose of this lab was to investigate the effect of sucrose concentration in water on the change in mass the potato chips. Drop disc into peroxide solution and start timer. The baked potato contained the second most amount of catalase with its average percent change in mass of 5. This means that the water molecules move out of the membrane, go into the membrane, or remain equal. SleestaksRule Recommended for you. This enzyme breaks down hydrogen peroxide, which is a harmful by-product of the process of cellular respiration if it builds up in concentration in the cells. Catalase lab report 1. Lab 2: Enzyme Action (revised Fall 2009) Experiment 6B: Biology with Vernier Lab 2 - Biol 2 1-Page of 24 Lab 2. To the first tube, add a small piece of potato. We will put the potatoes into different temperatures and pHs, and mash the potato to see how the activity of catalase changes. This enzyme. 1021/ja1110706. Catalase is not released by the pancreas: it occurs in most cells to break down toxic hydrogen peroxide, the by-product of various biochemical reactions. The decomposition of hydrogen peroxide by catalase is regarded as involving two reactions, namely, the catalytic decomposition of hydrogen peroxide, which is a maximum at the optimum pH 6. This enzyme breaks down hydrogen peroxide, which is a harmful by-product of the process of cellular respiration if it builds up in concentration in the cells. Investigating the effect of pH on the rate of catalase activity pH affects the rate of enzyme activity. Introduction. Catalase is a specific type of enzyme that breaks down Hydrogen Peroxide into water and oxygen. However, you are really asking two different questions. used up in the process. AP BIOLOGY LAB: Enzyme Catalysis? the question is " you may have observed the reaction of naturally-occurring catalase in tissue from either liver or potato. University. LETTER TO THE EDITOR Open Access A hypothesis on chemical mechanism of the effect of hydrogen Penghui Shi1,3*†, Wancang Sun1† and Pengzhong Shi2 Abstract Many studies have shown that hydrogen can play important roles on the antioxidant, anti-inflammatory and other. An Enzyme Controlled Reaction-Catalase 1. Your problem statement and hypothesis are extremely easy to write if you have determined your independent and dependent variables. One molecule of catalase can catalyze the decomposition of approximately 4 x 107 molecules H 2O 2 per second! In this lab activity, you will be using yeast catalase, but you could also use catalase from potatoes, carrots, plant leaves, chicken liver, or steak…. Did a catalase producing hydrogen peroxide lab, used manganese dioxide, fresh frozen liver, potato, ice, fine clean sand, etc. Mathematically, the data that we collected refutes our hypothesis, which said that the red-skinned potatoes would have the most catalase and the highest average bubble height. An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen. Catalase is a liver enzyme, and a powerful enzyme similar to glutathione*. Use the following key to record catalase activity: Catalase Activity Key Result Record No bubbling 0. On the appropriate data sheet, form a hypothesis for the two variables named above. In this lab, you will study an enzyme that is found in the cells of many living tissues. If soap is added to the hydrogen peroxide before the reaction, when the reaction happens, the oxygen gas. However, it is toxic to our cells. In Bio, we did a potato diffusion/water potential lab. Having three different substances allowed us to determine whether each contained catalase. Catalase accelerates the breakdown of hydrogen peroxide into water and oxygen. One function of catalase within cells is to prevent the accumulation of toxic levels of hydrogen peroxide (H 2 O 2 ) formed as a byproduct of metabolic processes. AP Biology 2004-2005 Lab 5: Cellular Respiration ESSAY 1990 The results below are measurements of cumulative oxygen consumption by germinating and dry seeds. Also this experiment is being done is because we want to further investigate about enzymes because enzymes control and effect almost every chemical. Liver Enzyme Lab Purpose : The purpose of this experiment was to determine the effect of the enzyme catalase found in liver on the decomposition of 6% hydrogen peroxide into water and oxygen gas. No, during Part A we took used liver and put it in more peroxide. Catalase Activity Lab. The purpose of this lab was to study what would happen to us if catalase didn't work as well as it does, if we had more acid in our system. Enzyme activity can also be affected by surface area, ie. Decision: * Hypothesis proven or non ( 1 sentence ) * Future experiments covering with H peroxide and catalase in life beings ( workss. The general procedure for the lab is outlined below, and specific details for each variable follow. Background Information. Our hypothesis was essentially a correct prediction-that the frozen potato would have a higher level of the enzyme catalase (and for the correct reason). Pearson, as an active contributor to the biology learning community, is pleased to provide free access to the Classic edition of The Biology Place to all educators and their students. Do type it, and try to use Excel or some other program to make your graphs if possible. Plants and some bacteria also produce amylase. Observe the catalyzed reaction of H2O2 and O2 by peroxidase enzyme. 5 ml, 5 ml, 7. Projects in the lab include: Conservation and Evolutionary Genomics We have used next-generation technologies to decipher the genome of the Mojave desert tortoise ( Gopherus agassizii ), a hallmark species of the desert southwest that is threatened with habitat loss and disease. The results obtained matched the hypothesis which stated that 37c ? is the optimum temp for enzyme catalase to break hydrogen peroxide which is a toxic product into water & oxygen. Write a hypothesis on your lab sheet about how temperature effects catalase. The catalase in potatoes breaks down hydrogen peroxide (H 2 O 2) into water and oxygen. Catalase Activity Lab. One thing I did not test for was the difference in the types of catalase that were available, which. Since there are five bars for each trial, we made each one a different color and included a key. Through Part A and B, we discovered that liver is more reactive than potato, and catalase is not reusable. Catalase has one of the highest turnover rates of all enzymes. Annie Chiang. This activity allows students to quickly investigate a ubiquitous enzyme, catalase, and the decomposition of hydrogen peroxide. It is important to have catalase in animal cells because without it, humans would die. Catalase lab - Enzyme Optimization: Students will analyze background information, develop a hypothesis, and design and carry out an experiment to determine optimum pH, concentration, temperature, or inhibitor for catalase in yeast. However, it is toxic to our cells. Effect of Environmental Variables on Enzyme Reaction Rate in Living Cells. Part 3 - The experiment supported the hypothesis: Catalase was reusable and the hydrogen peroxide wasn't reusable. subtilis and the other half E. Catalase works by the following mechanism: 2 H 2 O 2----->2 H 2 O + O 2. In conclusion, the raw potato contained the most catalase because the average percent change in mass of 9. The main cell structures are easy to see when viewed with the. In this experiment the catalase was used as control, it was derived from potato juice, The enzyme in this experiment was catalase, which was extracted from potato and liver. The graph somewhat supports the hypothesis presented in the introduction. Hypothesis. The organelle responsible for destroying hydrogen peroxide is the peroxisome using the enzyme catalase. Introduction. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase is an enzyme responsible for the degradation of hydrogen peroxide. Lab Report on Measuring the Rate of Conversion of Hydrogen Peroxide using Enzyme Catalysis 664 Words | 3 Pages. Catalase and peroxidase are heme enzymes that catalyze reactions of hydrogen peroxide. Although our hypothesis was primarily correct in that hydrogen peroxide would have the longest reaction time, we did not expect for ammonia and vinegar to have absolutely no. Conclusion. a) Describe the effect of damaging the potato on the catalase activity. ) We decreased the amount of substrate for each reaction assuming that the result would increase the reaction rate of the reaction. It speed up a reaction which breaks down Hydrogen Peroxide (H 2 O 2 ) into 2 harmless substances—water and oxygen. Our Experiment and Hypothesis Science! We designed and conducted an experiment to determine if the amount of potato (catalase) would affect the amount of oxygen released in the form of bubbles, when it was added to hydrogen peroxide. Pre-Lab Homework for Lab 5: Enzymes & Diffusion After reading over the lab, answer the following questions. Note that value may be positive (0. The potato is cut into 8 small cubes of 5mm x 5mm x 5mm. Conclusion: Write a one paragraph summary of the lab. Since our potato pieces are open to the atmosphere the Ψ P = 0. The foam produced is a result of catalase converting hydrogen peroxide into water and oxygen, the bubbles are filled with this oxygen. lt is possible for you to prop but all must be testable' Prediction Predict the result of experiment based on youl hypothesis. He then explains how you can measure the rate of an enzyme mediated reaction. Therefore, your cells. In Part D, we approached understanding the effect of different pH levels on the liver's catalase. Independent Variable: Temperature. It is a tentative explanation for an observation, phenomenon, or scientific problem that can be tested by further investigation. ) One unit of pure catalase decomposes 1 ɥmole H2O2 per minute at 25°C at pH7. We're using potato juice as the source for our catalase, having the students dip tiny disks of filter paper in them, then put them in hydrogen peroxide of various concentrations and timing how long until the disks rise to the surface. The New Fuss About Potato Enzyme Lab Report. Introduction Enzymes work as catalysts, which are substances that speed up chemical reactions. Directions: Complete the problems below by filling in the blank spaces. He also explains how temperature and pH could affect the rate of a reaction. Academic year. Hydrogen peroxide is a normal by-product of cellular metabolism; however, it quickly becomes a poisonous toxin if it builds up in the cell. Background Information: Hydrogen peroxide is a by-product of many biological functions of organisms and is inside our cells. 0 AIM: To study the rate at which catalase produces oxygen in various concentrations of hydrogen peroxide, using a pureed potato. 04 Turgor in potato tissue - preparation All zipped. The second part of the lab we tested out different substances such as potato, apple, and chicken to see if they contained catalase and how great of a reaction they would have with hydrogen peroxide. Then, we will see if there is a similar catalase present in potatoes. It speed up a reaction which breaks down Hydrogen Peroxide (H 2 O 2 ) into 2 harmless substances—water and oxygen. In this lab experiment, three different chemicals, each with a different pH level was used to check the effect of pH in the catalase activity. 2 Potato cubes Razor blade Procedure 2: Effect of enzyme concentration For this experiment you will use potato as a source of catalase. Cut away from the body. Before toy start this section, write the section title, purpose and a hypothesis on your lab paper. Fuller's webpage for online resources such as class notes, Powerpoints and animations. 6 Root pressure Glass tubes are fitted to the cut branches of a potted plant. After a problem is identified, the scientist would typically conduct some research about the problem and then make a hypothesis about what will happen. Hydrogen peroxide is a normal by-product of cellular metabolism; however, it quickly becomes a poisonous toxin if it builds up in the cell. Hypothesis #2 (The effect of a base on Catalase activity): If Catalase. Potato catalase lab hypothesis keyword after analyzing the system lists the list of keywords related and the list of websites with related content, in addition you can see which keywords most interested customers on the this website. Name: _____ AP Biology – Lab 05 Page 3 of 7 In this experiment, you will study the enzyme peroxidase obtained from turnips. The hypothesis that at temperatures above 40°C, the enzyme catalase will become less effective, is proven to be true. Design a simple experiment to investigate how catalase activity in potato is affected by pH. Home; Publications; News; PANIC Laboratory Research Projects. You should allow about two to three hours for the potatoes to soak in the water. Do type it, and try to use Excel or some other program to make your graphs if possible. Catalase has one of the highest turnover numbers of all enzymes; one molecule of catalase can convert millions of molecules of hydrogen peroxide to water and oxygen per second. Hypothesis: If the enzyme catalase is placed in different solutions containing different pH levels, the enzyme will function most effectively at a neutral pH, or a pH of 7. Subsequently, one potato piece is put in each of the beakers for approx. Testing the catalase reaction 1. The New Fuss About Potato Enzyme Lab Report. The enzyme catalase speeds up the breakdown of hydrogen peroxide (H202) into water (H2O) and oxygen gas (02). What is can be the hypothesis of this experiment?. Almost all chemical reactions that occur in living things are catalyzed by enzymes. Osmosis potato lab report - Online Student Writing Company - Get Help With Non-Plagiarized Essays, Research Papers, Reviews and Proposals Quick. This is possible because when a cell dies, the enzymes remain intact and active for several weeks, as long as the tissue is kept refrigerated. Hypothesis: Water potentials (Ψ w) of the potato tuber cells will be negative and should range from -0. The name of the enzyme is catalase; it speeds up a reaction which breaks down hydrogen peroxide, a toxic chemical, into 2 harmless substances--water and oxygen. After a problem is identified, the scientist would typically conduct some research about the problem and then make a hypothesis about what will happen. Purpose The purpose of this experiment is to find out the effects of pH upon the enzymes of the action between H 2 O 2 the enzyme catalase found in potatoes. Almost all chemical reactions that occur in living things are catalyzed by enzymes. The type of potato with the highest average bubble height was Idaho. Background information. Many bacteria scavenge hydrogen peroxide with a larger catalase, shown in the center from PDB entry 1iph , that uses a similar arrangement of iron and heme. Hydrogen peroxide is the substrate for catalase. Number the potatoes as one and two. In this lab, you will study the enzyme catalase, found in potato cells. Catalase is an enzyme found in food such as potato and liver. Conclusion In our hypothesis we predicted that due to the temperature, the catalase and hydrogen peroxide would have a slower reaction in the frozen and raw potatoes; due to higher temperatures in the boiled and baked, the catalase and hydrogen peroxide would have a faster reaction. Put soaked potato pieces into test tubes, one for ammonia, one for vinegar, and one for water for 90 seconds. Use the following key to record catalase activity: Catalase Activity Key Result Record No bubbling 0. Hypothesis. Through this lab we explored how catalase reacts with different substances and how change in temperature affects the reaction rate. Fortunately, there’s an enzyme which helps neutralize oxidative compounds like hydrogen peroxide. In experiment number 3, we are comparing the action of catalase in different tissues by testing this hypothesis: The breakdown of hydrogen peroxide occurs at the same rate in all cells. In this lab experiment, three different chemicals, each with a different pH level was used to check the effect of pH in the catalase activity. Disclaimer and Safety Precautions Education. The ground-up potato is filtered through cheesecloth to separate potato skin and cell debris from the liquid which contains most of the cell's enzymes, including catalase. Osmosis Lab Introduction : Human blood, at 0. Powered by Create your own unique website with customizable. enzyme called catalase breaks down hydrogen peroxide into water and oxygen. Repeat steps 7&8. The reaction for the break-down of hydrogen peroxide (by catalase) into water and oxygen gas is given below. Potato Polyphenol Oxidase (PPO) Assay and Data Analysis Introduction Potato polyphenol oxidase (PPO) is an enzyme that is activated upon injury to the potato, e. Enzyme Catalysis Lab Review Worksheet - Winnie Litten. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown in the equations below. In this lab, we will use a catalase solution from potatoes and determine the effect of temperature and pH on the action of this enzyme. If you put a potato into an isotonic solution it should remain the same weight, but if you have more solute in the water than in the potato, water from the potato will be drawn out (like salting a snail or tomato), but if the water has less solute than the potato, it will be drawn into the potato, making it heavier. Lab 1 - The Scientific Method and Termite Trails; Lab 2 - Animal Behavior and The Betta Lab; Lab 3 - Diffusion and Osmosis and The Kidney Dialysis Simulation Lab; Lab 4 - Enzymes and The Catalase Lab; Lab 5 - DNA Extraction From Cells; Lab 6 - Inheritance of Traits and the Dragon Genetics; Lab 7 - Gel Electrophoresis and DNA. In this experiment, you will study the naturally occurring catalase molecule found in potatoes. For example, potato waffles are stored in the freezer, but potatoes themselves. In the second part, you will observe plasmolysis in an aquatic plant, Elodea. Throughout this experiment we will look for any change in the mass of the potato cylinders and therefore, will be taking quantitative measurements. The abstract is written single spaced, in a font smaller than the text for the body of the lab report, and the margins are justified. 9% salt concentration, is a little less salty than seawater , which has a salt concentration of about 35 parts per thousand (3.